Signal transduction: Regulation of the H+ pump
In our group we identify components of the signal transduction pathways leading to regulation of the H+ pump.
The plasma membrane H+-pump is an essential enzyme being involved in developmental processes as well as responding to external physiological stimuli. This requires a tight coordination of protein kinases and phosphatases regulating the activity of the pump by phosphorylation events.
A wide range of techniques are employed in order to fully understand the processes taking place at the cellular level in the plant: molecular biology, generation and characterization of mutants, heterologous expression of proteins, protein-protein interaction, enzyme activity, bioimaging and proteomics.
Yang Y, Qin Y, Xie C, Zhao F, Zhao J, Liu D et al (2010). The Arabidopsis chaperone J3 regulates the plasma membrane H+-ATPase through interaction with the PKS5 Kinase. Plant Cell 22(4):1313-1332
Liu J, Elmore JM, Fuglsang AT , Palmgren M, Staskawicz BJ, Coaker G (2009). RIN4 functions with plasma membrane H+-ATPases to regulate stomatal apertures during pathogen attack. PLoS-Biology 7(6)
Fuglsang AT, Guo Y, Cuin TA, Qiu Q, Song C, Kristiansen KA et al (2007) Arabidopsis protein kinase PKS5 inhibits the plasma membrane H+-ATPase by preventing interaction with 14-3-3 protein. Plant Cell 19(5):1617-1634
Niittyla T, Fuglsang AT, Palmgren MG, Frommer WB, Schulze WX (2007). Temporal analysis of sucrose-induced phosphorylation changes in plasma membrane proteins of Arabidopsis. Molecular and Cellular Proteomics 6(10):1711-1726