High throughput polysaccharide profiling

We have developed microarray-based technology for the high throughput mapping of cell wall components. The technique ‘Comprehensive Microarray Polymer Profiling’ or CoMPP combines the high throughput capacity of microarrays with the specificity of monoclonal antibodies. Using CoMPP, the relative abundance of cell wall epitopes can be rapidly assessed across large sets of samples - for example, different plant species, organs, tissues, developmental stages, mutants, or processing steps. Polysaccharides are sequentially extracted from cell walls, spotted as microarrays, probed with monoclonal antibodies  or carbohydrate binding modules and spot signals are quantified. Analysis can be can be performed on less than 1 mg of cell wall material (alcohol insoluble residue) and several hundred samples can be processed simultaneously.

 

 

Selected publications

Moller, I., Sørensen, I., Bernal, A.J., Blaukopf ,C., Lee, K., Øbro, J., Pettolino, F., Roberts, A., Mikkelsen, J., Knox, J.P., Bacic, T. and Willats W.G.T (2007) High-throughput mapping of cell wall polymers within and between plants using novel microarrays. The Plant Journal 50(6): 1118-28