Carbohydrate microarrays

We have developed carbohydrate microarray technology for a wide range of applications. Arrays of glycans extracted from plant materials are used for the high throughput mapping of cell wall polysaccharide populations across a range of samples. We also use arrays of well defined oligosaccharides for the: detailed analysis of antibody and carbohydrate binding modules and high throughput screening of mAbs and CBMs; glycan ligand discovery and high throughput enzyme characterisation. We are equipped with two microarray robots, one based on pin technology (MicroGrid II, BioRobotics) and one that uses a piezoelectric print head (Sprint, ArrayJet). We produce arrays on range of surfaces including glass and plastic slides as well as membranes.

 

Selected publications 

Øbro, J., Sørensen, I., Derkx, P., Madsen, C.T., Drews, M., Willer, M., Mikkelsen, J.D. and Willats W.G.T. (2008) High-throughput screening of Erwinia chrysanthemi pectin methylesterase variants using carbohydrate microarrays. Proteomics, 9(7), 1861-1868.

 

Sørensen, I.,  Pedersen, H.L. and  Willats, W.G.T. (2008) An array of possibilities for pectin. Carbohydrate Research, doi:10.1016/j.carres.2008.12.008

 

Moller, I, Marcus, S.E., Haeger, A., Verhertbruggen, Y., Verhoef, R., Schols, H., Ulvskov, P., Mikkelsen, J.D., Knox, J.P. and Willats, W.G.T. (2007) High-throughput screening of monoclonal antibodies against plant cell wall glycans by hierarchical clustering of their carbohydrate microarray binding profiles. Glycoconjugate Journal 25: 37-48.

 

Øbro, J., Sørensen, I., Moller, I., Skjøt, M., Mikkelsen, J. and Willats, W.G.T. (2007) High-throughput microarray analysis of pectic polymers by enzymatic epitope deletion Carbohydrate polymers 70(1): 77-81.

 

Willats, W. G.T., Rasmussen, S. E., Kristensen, T., Mikkelsen, J. D. and Knox, J. P. (2002) Sugar-coated microarrays: a novel slide surface for the high throughput analysis of glycans. Proteomics 2(12): 1666-1671.